The F-box protein 8 Gene (FBXO8) has been shown to suppress invasion and metastasis in various cancer types. Recurrence and drug resistance pose significant challenges in renal cell carcinoma (RCC). Identifying novel biomarkers is crucial for addressing these issues.
Data on RNA sequencing and patient survival for KIRC was obtained from The Cancer Genome Atlas (TCGA), UALCAN, and Gene Expression Omnibus (GEO) databases. We confirmed FBXO8 gene expression and its impact on survival. Clinical characteristics were classified, and FBXO8 expression differences among various categories were observed. We conducted biofunctional predictions and analyzed the tumor microenvironment (TME), immune cell infiltration, and immune checkpoints in relation to FBXO8 expression. FBXO8 was overexpressed using a plasmid, and we assessed Kidney renal clear cell carcinoma (KIRC) cell proliferation, migration, and apoptosis through CCK8, wound healing tests, and western blot analysis.
Our findings revealed decreased FBXO8 expression in KIRC, with patients exhibiting low FBXO8 expression experiencing shorter survival times. The low expression group showed elevated TME immune and estimate scores. Biofunctional analyses indicated that FBXO8 expression was notably linked to drug metabolism cytochrome P450, nutrition disease, receptor-ligand activity, and neuroactive ligand-receptor interaction. Furthermore, we discovered significant correlations between FBXO8 expression and immune cell infiltration, as well as checkpoints such as CD274. Overexpression (OE) of FBXO8 led to a marked reduction in cell proliferation and migration, along with increased apoptosis, as evidenced by apoptosis-related protein expression.
This study demonstrates that FBXO8 serves as a biomarker for KIRC and plays a role in regulating cell proliferation, migration, and apoptosis.
Cancer genetics. 2024 Nov 09 [Epub ahead of print]
Zhouan Luo, Xiaoping Wu, Juanxia Xie, Hao Tang, Jingqi Chen, Dongcai Ye, Shangwen Dou, Songning Chen
Department of Urology, Zhanjiang Central Hospital, Guangdong Medical University, No.236, Yuanzhu Road, Zhanjiang, Guangdong, 524045, China., Department of Geriatrics, Zhanjiang Central Hospital, Guangdong Medical University, China., Department of Rehabilitation Medicine, Zhanjiang Central Hospital, Guangdong Medical University, China., Department of Urology, Zhanjiang Central Hospital, Guangdong Medical University, No.236, Yuanzhu Road, Zhanjiang, Guangdong, 524045, China. Electronic address: .